Salkowski test (for cholesterol)

Salkowski test (for cholesterol)

Principle:

Salkowski test is used to detect cholesterol in a solution. This test is named after a German biochemist Ernst Leopold Salkowski.  Cholesterol solution in chloroform when shaken with equal volumes of concentrated sulphuric acid and layers allowed to separate by standing, the chloroform layer turns red.

Reagents:

  • Cholesterol solution
  • Chloroform
  • Concentrated Sulphuric acid

Sample preparation:

  • Separate the egg yolk (yellow part) of a boiled egg and grind it with about 50 ml of chloroform in a mortar.
  • Filter the suspension through a filter paper.
  • The filtrate is the cholesterol solution. The egg yolk is the rich source of cholesterol.

Procedure:

  • Take 1 ml of cholesterol solution in chloroform in a dry test tube.
  • Add 1 ml of concentrated sulphuric acid. The two layers do not mix.
  • Shake the tube to mix the two layers.
  • The upper layer i.e. chloroform layer turns red.
  • The lower layer i.e sulphuric acid layer turns yellow colour with green fluorescence.

Interpretation:

  • The upper layer consisting of chloroform becomes bluish-red to cherry-red, whereas the layer of sulphuric acid becomes yellow with green fluorescence.

 

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